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Doncaster captain Rob Jones' injury absence has been prolonged after the defender suffered a setback in training.
Two goals from powerful striker Luke Norris secured Gillingham a 2-1 success at Doncaster and their first league win on the road.
Paul Dickov said Doncaster needed to keep believing after a 2-1 home defeat to Gillingham in a game they had dominated.
Doncaster have extended Enda Stevens' loan spell from Aston Villa for another month.
Doncaster boss Paul Dickov had no regrets about changing a winning team after Rovers were knocked out of the Johnstone's Paint Trophy.