Ainsworth’s side welcomed Morecambe to Adams Park knowing victory could see them place one foot into League One.
However, Paul Mullin’s late header earned the visitors all three points and – after Southend and Bury both won – they head into the final day of the season in fifth, three points off promotion.
“We have to rely on two games on the last day of the season away from home, think I’ve been there before,” said Ainsworth. “But we’ll see what happens.
“No matter what happens next week it’s still in our hands because if we fall in those play-offs, if you win three games you’re promoted. But next week I will be going for the win because we need a win to get above the other two.
“We need favours from other teams but I’ll be hoping that happens, and if it doesn’t then we readdress where we are and we go again.
“Obviously I’m disappointed we’ve lost the game, we didn’t do enough going forward to win the game and we didn’t defend set plays well at all.”
Morecambe were the better team for much of the afternoon at Adams Park and Wycombe had goalkeeper Matt Ingram to thank on more than one occasion.
The 21-year-old denied Jack Redshaw, Alfie Mawson, Alex Kenyon, Andy Parrish and Kevin Ellison in a superb display but Mullin ensured four minutes from time it was all in vain.
“We’re very pleased,” said Morecambe boss Jim Bentley.
“We knew we were coming into a big occasion today for Wycombe but we’re professionals, we want to win every game and it will be exactly the same next week when we play Southend so we’re going to play some part in the promotion race but it was a great performance from us.
“There was not much between us both and I thought they took a few chances because they had to as they knew Southend were winning.
“We hit them on the break a couple of times and our set plays were excellent and only for the keeper pulling off some outstanding saves we probably should have been one or two goals up before we got the all important one.
“We’re very pleased, it was a solid away performance and we’ve been good away from home this season. We set ourselves aims and targets and we want to get to 60 points.”