Barker is ruled out with a thigh injury, which also kept him out of Saturday’s draw with West Brom.
The 27-year-old is also expected to miss Saturday’s clash with Watford at Vicarage Road.
The injury to Barker means former Blackburn defender Jay McEveley will deputise once again at centre-half.
However, Clough has better news regarding the fitness of midfielder Paul Green.
Green has been sent for a precautionary scan after complaining of tight hamstrings but will travel with the team.