Premier League side Saints will face the Bees on July 22 at Griffin Park, which is just a few miles from the site of Wednesday’s horrific events.
Brentford chief executive Mark Devlin said: “Firstly, I would like to add the sincere condolences of the club to those who have lost family and friends, our thoughts and prayers are with them.
“The club is situated relatively close to the site of the disaster, and we wanted to do something to support our community and those affected.
“Brentford is very much a community club and I’m delighted that Southampton have joined with us in donating the profits from the ticket sales of our friendly match.
“We also anticipate there will be other fundraising activities on the day and we hope that as many fans as possible will be able to attend the game, so that we can raise as much as possible.”