Brentford will be without Karleigh Osborne, Kevin O’Connor and Jake Bidwell for the home fixture.
Centre-half Osborne is back in training after a month out with a foot injury, but will step up his comeback with a reserve outing next week.
Long-serving midfielder O’Connor is likely to be out for a couple of months with the foot injury sustained in the first-round win over Basingstoke.
Young defender Bidwell does not have permission to feature in the competition as parent club Everton do not want him cup-tied, but fellow loanee Harlee Dean has been given the green light to play by Southampton.
Teenage forward Emmanuel Oyeleke could be added to the bench with seven substitutes allowed.