Cheltenham are injury and suspension-free as they make the trek to the non-league club.
The Robins are still waiting for permission to play for loanees Luke Garbutt, James Spencer, Jack Butland, Bobby Reid and Marlon Jackson from the players’ respective parent clubs.
Otherwise, manager Mark Yates and his in-form squad have had the ideal preparation for the trip to Kenilworth Road.
Striker Darryl Duffy, who netted the winner in the 1-0 victory at Tranmere in round one, is likely to keep his place up front.
Duffy is expecting a tough tie, telling Press Association Sport: “We’re not going to treat it lightly and we’ve got to show the same kind of attitude and determination that we did when we went to Tranmere.”