Mackie, who has scored four goals for Argyle in the Championship this term, missed Saturday’s 1-0 home defeat by Sheffield United following his one-match ban for receiving five cautions.
Boss Paul Sturrock partnered World Cup-bound New Zealand international Rory Fallon in attack with recalled 20-year-old striker Ashley Barnes.
Sturrock also used the game to introduce 18-year-old forward Joe Mason.
Mason made his debut on the right of midfield while, as anticipated, fit-again central defender Kari Arnason returned in the back four and should keep his place.
Right-back David Gray – on loan from Manchester United – was also recalled and is also expected to stay in the side.