Naylor, who only returned to the side three games ago following a month’s lay-off due to a calf injury, was forced out of the action in the 80th minute in the Boxing Day win over Hartlepool.
If he fails to make it, Lubo Michalik is ready to step in to partner Patrick Kisnorbo at the heart of defence.
Andrew Hughes is another injury doubt for Leeds after he sustained a knock against Hartlepool, so Bradley Johnson and Tony Capaldi, on loan from Cardiff, are on stand-by.
Manager Simon Grayson, who has no other new injury or suspension problems, must decide whether to recall on-loan QPR midfielder Hogan Ephraim, left out of the matchday squad altogether on Boxing Day, as Leeds look to extend their unbeaten league run to 10 matches.
Meanwhile, Grayson was delighted with the response shown by Jermaine Beckford in the 3-1 victory over Pools.
The striker, who took his season’s tally to 17, was forced to apologise to Grayson during the week after reacting angrily to being substituted against Southampton last week.
Armann Bjornsson had put Hartlepool in front, but Beckford netted either side of Luciano Becchio’s strike to give Leeds all three points at Elland Road.
Grayson said: “Everyone is pleased for Jermaine. It wasn’t his day against Southampton but this was his day.
“He was in the right place at the right time for his first goal, he hit the bar and for his second goal he worked their keeper well.”