The home side came closest to breaking the deadlock when Marcus Bent hit the upright early on. Bent headed Jason Koumas’ corner against the post.
After a dull first half, the restart saw a much higher tempo with Royals goalkeeper Marcus Hahnemann first tipping over Michael Brown’s rising drive and then Emile Heskey sending a powerful header wide from the resulting corner.
Reading also appeared energised by the break and Liam Rosenior claimed his header at the far post had been handled before Wigan cleared.
Dave Kitson had two late chances to put the visitors in front but it finished goalless.
Reading stay 17th, a point above Birmingham with two games left, while Wigan stay 13th, four points clear of danger.