Luke Shaw withdrew from the England squad for Sunday’s World Cup qualifier in Slovakia after missing training on Saturday morning.
The 21-year-old left-back has only just returned from double leg-break and has started the season well with Manchester United.
He was replaced in training at St George’s Park by Arsenal’s Tafari Moore, who is part of the Under-20s set-up, and did not fly with the team.
Shaw now misses out on a potential England return having last been capped almost a year ago in a 2-0 Euro 2016 qualifying victory over Switzerland at Wembley.
Shaw was not due to start in manager Sam Allardyce’s first game in charge, with Tottenham’s Danny Rose preferred at left-back.
The Football Association confirmed on Saturday evening that Shaw would not travel to Slovakia.
“Luke Shaw has left the England camp and returned to Manchester United,” a statement read.
“The left-back was rested from training at St George’s Park this morning, with the medical team continuing to manage his workload.
“With Danny Rose set to start against Slovakia in Trnava tomorrow night, Shaw has been allowed to return to Manchester to train with his club as a precautionary measure.
“Sam Allardyce’s remaining 22 players all travelled out to Slovakia this afternoon.”
While it is currently unclear if Shaw faces a spell on the sidelines, any injury will be seen as a blow as the former Southampton youngster looks to rebuild his career after missing so much football after breaking his leg in a Champions League tie against PSV Eindhoven last year.
Speaking earlier in the week Shaw said he was not squeamish about the injury and was happy to show off the scars left behind by the surgery.
”I don’t mind answering questions about my leg. I think sometimes people don’t like talking about it, but I’m not bothered,” he said.
”I wasn’t bothered watching videos of it or pictures of my leg. That might sound a bit weird, but I feel comfortable talking about it because now I feel stronger than ever.
”At first I did start thinking, ‘Am I really like this?’, but it’s my leg at the end of the day.
”Obviously I went through a bad time during the leg break, but I came through that stronger as a person. Now it does not faze me.”