Bournemouth midfielder Jack Wilshere and Spurs striker Harry Kane have been included in the England squad for the upcoming games against Scotland and Spain.
Adam Lallana, Raheem Sterling and Nathaniel Clyne also return after injuries forced them to miss the games against Malta and Slovenia.
Michael Keane retains his place in the squad as his search for a first England cap continues, as the Three Lions look ahead to their qualifier against Scotland on Friday night.
“Over the last few weeks we’ve been looking at as many games as possible and keeping track of all of the players,” Southgate told the official FA website.
“There were some players that weren’t available to us last time with injury who are in good form that make the frame – the likes of Nathaniel Clyne, Adam Lallana and Raheem Sterling. It’s good to be able to welcome them back.
“Similarly Harry Kane. We had some good discussions with a lot of the clubs over the past few weeks, and Tottenham have been very helpful in terms of Harry’s availability. I’m grateful to Mauricio [Pochettino] for that.”
Kane has missed seven weeks with an ankle injury but started the north London derby against Arsenal on Sunday and scored from the penalty spot to secure a 1-1 draw.
“Of course, Jack Wilshere is a player that we feel has a lot of class and is now starting to get more 90-minutes under his belt. It’s good to bring him back into the squad.”
Wilshere has been a conspicuous absentee from international get-togethers since Euro 2016, with the departed Sam Allardyce and his temporary replacement both leaving him out.
But a loan move from Arsenal to Bournemouth has given the 24-year-old some much-needed game time and he has rebuilt both form and fitness under Eddie Howe.
Manchester United pair Chris Smalling and Luke Shaw are not involved in a 25-man squad after being declared unfit for the 3-1 win over Swansea – a decision that seemed to frustrate their club boss Jose Mourinho.With Smalling missing, Burnley’s Michael Keane edges Middlesbrough’s Ben Gibson – the former having received his first call-up as injury cover for last month’s games.
Goalkeepers: Fraser Forster (Southampton), Joe Hart (Torino, loan from Manchester City), Tom Heaton (Burnley),
Defenders: Ryan Bertrand (Southampton), Gary Cahill (Chelsea), Nathaniel Clyne (Liverpool), Phil Jagielka (Everton), Michael Keane (Burnley), Danny Rose (Tottenham Hotspur), John Stones (Manchester City), Kyle Walker (Tottenham Hotspur)
Midfielders: Eric Dier (Tottenham Hotspur), Danny Drinkwater (Leicester City), Jordan Henderson (Liverpool), Jack Wilshere (AFC Bournemouth, loan from Arsenal), Adam Lallana (Liverpool), Jesse Lingard (Manchester United), Wayne Rooney (Manchester United), Raheem Sterling (Manchester City), Andros Townsend (Crystal Palace), Theo Walcott (Arsenal)
Strikers: Harry Kane (Tottenham Hotspur), Marcus Rashford (Manchester United), Daniel Sturridge (Liverpool), Jamie Vardy (Leicester City)