The Bees' slim play-off hopes were finally ended after they were beaten 2-1 at home to promotion-chasing Sheffield Wednesday on Saturday.
But despite having little to play for next weekend, Rosler has vowed to field his strongest XI.
"I will put out the best team I can at Chesterfield, there will be no free tickets," he said.
"I want to go there and win. Everyone will have the chance this week to put themselves in a position to play."
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