James Collins and Jack Collison are out of the Wales squad to face Bosnia-Herzegovina, with Joel Lynch and Robert Earnshaw replacing them.
Collins, who rejoined West Ham in the summer from Aston Villa, has withdrawn from Wednesday's friendly with a groin injury.
West Ham midfielder Collison has been forced out with a knee problem.
Wales boss Chris Coleman has called up Huddersfield defender Lynch and Cardiff striker Earnshaw as replacements in his squad.
Team GB quartet Craig Bellamy, Aaron Ramsey, Joe Allen and Neil Taylor are all included in the squad.
Wednesday's game is Coleman's final chance to prepare his squad for the forthcoming World Cup qualifiers.
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