Ricky Holmes is set to return to the Barnet squad for the clash.
The striker, who has scored six goals this season, has been out for a month with a knee injury but has trained all week.
On-loan QPR full-back Gary Borrowdale is also fit after a groin problem and pushing to be involved.
But the Bees are still without defensive duo Darren Dennehy and Daniel Leach and strikers Charlie Taylor and Steve Kabba.
Dennehy has not recovered from a calf niggle in time, Leach has a tendon injury, Taylor a twisted ankle and Kabba a long-term knee problem.
Boss Lawrie Sanchez will also be without Lincoln loanee Gavin McCallum, who is cup-tied.
MK Dons boss Karl Robinson will make a host of changes for the trip.
Midfielder Darren Potter will be one of several first-team regulars to be rested against the League Two club, who are managed by Wimbledon legend Lawrie Sanchez.
Mark Beevers is unavailable under the terms of his loan from Sheffield Wednesday, so Gary MacKenzie will be given the chance to stake a claim in defence after losing his place in the team.
Shaun Williams might also get a run-out while fellow midfielder George Baldock and defender Adam Chicksen could also be involved.
As he did for the first-round tie against non-league Nantwich, Robinson will include a handful of teenagers in the squad. Leon Lobjoit, 17, George Williams, 16, and Brendan Galloway and Dele Alli, who are both 14, are in the running to be on the bench.
Williams became the club’s youngest ever goalscorer when he netted Dons’ sixth goal against Nantwich.