Leeds midfielder Kalvin Phillips and Manchester United forward Mason Greenwood have earned their first call-ups in the latest England squad.
Meanwhile, Southgate has also included United defender Harry Maguire, amid his ongoing court case in Greece.
The Red Devils‘ captain is not present at his trial, where he is facing charges relating to an alleged bust-up with police.
There is also a first call-up for Manchester City star Phil Foden, who rose to further prominence under Pep Guardiola last season.
Danny Ings’ fine form for Southampton has seen him called up for the first time since making his debut in 2015. The Saints hitman netted 22 Premier League goals, his best return to date.
Kyle Walker, meanwhile, has been recalled having not played for England since the Nations League finals last June.
Here is your first #ThreeLions squad of the year! 🙌
Gareth Southgate has named a 24-man squad for next month’s #NationsLeague games against Iceland and Denmark.
— England (@England) August 25, 2020
City team-mate John Stones has been left out of the squad, but Raheem Sterling got the the nod despite questions over his availability.
The winger attended a party with Usain Bolt – who has reportedly tested positive for coronavirus. However, Sterling has since posted a negative test.
There is no place for Callum Hudson-Odoi, Fikayo Tomori, Danny Rose and Callum Wilson. They were all in the squad to face Kosovo in November, while Ross Barkley and Fabian Delph are also conspicuous by their absence.
Jordan Henderson, Alex Oxlade-Chamberlain, Ben Chilwell and James Maddison miss out through injury.
England face Iceland and Denmark in the Nations League next month.